Dr.Sangeeta Negi

Associate Professor
Department of Biotechnology
Motilal Nehru National Institute of Technology Allahabad, Prayagraj, India-211004
E-mail: sn5[at]mnnit[dot]ac[dot]in
Telephone: 05322271234(O) 05322271710(R) 9415015811(M)

Research Publications

Patents:

1. Title “A PROCESS FOR BIOREMEDIATION of INDUSTRIAL GREASE WASTE USING A BACTERIAL CONSORTIUM”. Patent No: 330765, awarded on: 30 Jan 2020. 2. Title: A BIOPOLYMER COATING FOR IMPLANTS AND A METHOD OF PREPARATION THEREOF. Application No.: 202111015120 filed on 31 March 2021 3. Title: DETOX CANDLE FOR ON-SITE DETOXIFICATION OF LIGNOCELLULOSE HYDROLYSATE AND METHOD OF FABRICATION THEREOF. Application No.: 202111033291 filed on 23July 2021

Books:

1.Editor of Book: Pretreatment of Biomass: Processes and Technologies, Edition: 2014, Publisher: Elsevier. 2. Editor of Book: Current Developments in Biotechnology and Bioengineering : Production, Isolation and Purification of Industrial Products’, ISBN 9780444636621. ELSEVIER B.V., offices at Radarweg 29, 1043 NX Amsterdam, The Netherlands (the “Publisher”)

Book Chapter:

1. Sangeeta Negi (2010), Cold-active enzymes in food industry, In-Comprehensive Food Fermentation Biotechnology, Editors- A Pandey et al., Asiatech Publisher Inc, New Delhi, Vol.1, pp134-152 2. Sangeeta Negi (2013), ISBN: 978-1-4614-8004-4 In book: Biotransformation of Agro-industrial waste: Fine Biochemicals, Edition:2014, Chapter14: Exploring plant and agro-industrial wastes for antimicrobial biochemicals Publisher: Springer Science+Business Media,New York USA2014., Editors- S K Brar & G S Dhillon, pp.335-366. 3. Sangeeta Negi and Siddharth Vats (2013), 01/2014; ISBN: 9789382332763 In book: Advances in Industrial Biotechnology, Edition: 2014, Chapter6: Biorefinery prospects: A study on pine litter feedstock, Publisher: I..K. International Publishing House Pvt.Ltd., Editors: Ram Sarup Singh, Ashok Pandey & Christian Larroche, pp.100-116. 4. Sangeeta Negi and Ajay Pandey(2014) In book: Pretreatment of Biomass: Processes and Technologies, Edition: 2014, Chapter5: Ionic liquid pretreatment. Publisher: Elsevier. 5. Sangeeta Negi and Vibha Kumari (2015) In book: Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products’. Under process. Agreement with ELSEVIER B.V., offices at Radarweg 29, 1043 NX Amsterdam, The Netherlands (the “Publisher”), Chapter2: Glucoamylases. 6. Sangeeta Negi (2017), In book: Biofuels, Green Energy and Technology, DOI 10.1007/978-981-10-3791-7_6 ., ©Springer Nature Singapore Pte Ltd. 2017, A.K. Agarwal et al. (eds.),Chapter 6: Pretreatment Strategies of Lignocellulosic Biomass Towards Ethanol Yield: Case Study of Pine Needles (Negi S. (2017) Pretreatment Strategies of Lignocellulosic Biomass Towards Ethanol Yield: Case Study of Pine Needles. In: Agarwal A., Agarwal R., Gupta T., Gurjar B. (eds) Biofuels: pp 85-103. Green Energy and Technology. Springer, Singapore). 7. Sangeeta Negi (2019) Book Chapt: Lipases: A promising tool for Food Industry in a book “Green Bio-Processes: Enzymes in Industrial Food Processing” edited by Vinod Parameswaran, Emmanuel Papamichael, Sunita Varjani, Sindhu Raveendran.

Journals:

1. Ajay K Pandey and Sangeeta Negi (2020) Enhanced Cellulase Recovery in SSF from Rhizopus oryzae SN5 and Immobilization for Multi-batch Saccharification of Soluble Carboxymethylcellulose. Biocatalysis and Agricultural Biotechnology. 26 (2020) 101656. https://doi.org/10.1016/j.bcab.2020.101656. 2. Kumari Vibha and Sangeeta Negi (2020) Formulation of Biocomposite of Ultrasonication Mediated Cellulose and Lignin Nanofibers for Biomedical Applications (Article Id: AIR-47). June 3. Sangeeta Negi, Sapna Jain, Ananad Kumar (2020) Combined ANN/ EVOP Factorial Design Approach for Media Screening for Cost-effective Production of Alkaline Proteases from Rhizopus oryzae (SN5) /NCIM-1447 under SSF. AMB Express.10:60. DOI: 10.1186/s13568-020-00996-7. Published: 27 March 2020 4. Kumari Vibha and Sangeeta Negi (2020) Enzymatic plasticising of lignin and styrene with adipic acid to synthesize a biopolymer with high antioxidant and thermostability. Polymer Degradation nad stability. 174 (2020) 109081. https://doi.org/10.1016/j.polymdegradstab.2020.109081. . 5. Vibha, K. & Negi, S (2018) Enhanced production of laccase and pectinase using co-culture of Trametes hirsuta and Phanerochaete sp. through EVOP-factorial design technique. 3 Biotech. 8: 490. https://doi.org/10.1007/s13205-018-1516-4. 6. Sunil Kumar, Sangeeta Negi and Pralay Maiti (2017) Biological and analytical techniques used for detection of polyaromatic hydrocarbons. Environ Sci Pollut Res. 24, pages25810–25827(2017). https://doi.org/10.1007/s11356-017-0415-2. 7. Arti Kumari, Razi Ahmad, Sangeeta Negi, Sunil Kumar Khare (2017) Biodegradation of waste grease by Penicillium chrysogenumfor production of fatty acid. Bioresource Technology.226, 31–38. 8. Ajay Kumar Pandey, Edgard.Gnansou and Sangeeta Negi (2016) Optimization of Concomitant Production of Cellulase and Xylanase from Rhizopus oryzae SN5 through EVOP-Factorial Design Technique and Application in Sorghum Stover Based Bioethanol Production. Renewable Energy. 98 pp.51-56. DOI: 10.1016/j.renene.2016.05.071. 9. Farhan Anjum, Gunjan Gautam, Gnansou Edgard and Sangeeta Negi (2016) Biosurfactant production through Bacillus sp. MTCC 5877 and its multifarious applications in food industry. Bioresource Technology. 213, 262–269. 10. Ajay Kumar Pandey and Sangeeta Negi (2015). Impact of surfactant assisted acid and alkali pretreatment on lignocellulosic structure of pine foliage and optimization of its saccharification parameters using response surface methodology. Bioresource Technology. 192 (2015) 115–125. 11. Devendra Prasad Maurya, Ankit Singla, Sangeeta Negi (2015) An overview of key pretreatment processes for biological conversion of lignocellulosic biomass to bioethanol.3 Biotech, 5(5): 597–609. 12. Sunil Kumar and Sangeeta Negi (2014) Transformation of waste cooking oil into C-18 fatty acids using a novel lipase produced by Penicillium chrysogenum through solid state fermentation. 3Biotech. DOI 10.1007/s13205-014-0268-z accepted on 20 November 2014. IF: 1.361 13. Gunjan Gautam, Vishwas Mishra, Payal Verma, Ajay Kumar Pandey and Sangeeta Negi*(2014) A Cost Effective Strategy for Production of Bio-surfactant from Locally Isolated Penicillium chrysogenum SNP5 and Its Applications. J. Bioproces Biotechniq 2014, 4:177, http://dx.doi.org/10.4172/2155-9821.1000177. 14. Sunil Kumar, Rajnesh Kumari Yadav and Sangeeta Negi (2014) A comparative study of immobilized lipase produced from Penicillium chrysogenumSNP5 on two different anionic carriers for its pH and thermostability. Indian Journal of Biotechnology.vol13:301-305. 15. Jayanti Kumari and Sangeeta Negi (2014) Optimization of process variables for laccase production from Pleurotus ostreatus through Evolutionary Optimization Techniques. Indian Journal of Experimental Biology. 52(11) 1106-1111. 16. Animeksh K. Ojha, Stefan Forster, Sumeet Kumar, Siddharth Vats, Sangeeta Negi and Ingo Fischer (2013). Synthesis of well-dispersed silver nanorods of different aspect ratios and their antimicrobial properties against gram positive andnegative bacterial strains. Journal of Nanobiotechnology. 11:42 17. Siddharth Vats, Devendra Prasad Maurya, Ayushi Jain, Varija Mall and Sangeeta Negi (2013), Mathematical model-based optimization of physico-enzymatic hydrolysis of Pinus roxburghii needles for the production of reducing sugars, Indian Journal of Experimental Biology.51,(11),944-953. 18. Devendra Prasad Maurya, Siddharth Vats, Sudheer Rai and Sangeeta Negi (2013), Optimization of enzymatic saccharification of microwave pretreated sugarcane tops through response surface methodology for biofuel, Indian Journal of Experimental Biology. 51,(11),992-996. 19. Siddharth Vats, Devendra Prasad Maurya, Ayushi agarwal, Mohmmad Shamoon and Sangeeta Negi (2013), Development of a microbial consortium for the production of blend of enzymes for the hydrolysis of agricultural wastes into sugars. Journal of scientific and Industrial Research, 72, 585-790. 20. Siddharth Vats and Sangeeta Negi. (2013) Use of Artificial Neural Network (ANN) for the development of bioprocess using Pinus roxburghii fallen foliage for the release of polyphenols and reducing sugars. Bioresource Technology, 140,392-398. 21. Wesley, Sunil Bala, Devendra Prasad Maurya, Hari Sharan Goyal, and Sangeeta Negi. (2013) Experimental investigation of microbiologically influenced corrosion of selected steels in sugarcane juice environment.World Journal of Microbiology and Biotechnology. 29:2353–2357. 22. Sangeeta Negi and Sunil Kumar (2012). Evaluation of techniques used for parameters estimation: an application to bioremediation of grease waste. Applied Biochemistry and Biotechnology, 167,1613–1621. 23. Sunil Kumar, Anisha Mathur,Varsha Singh, Suchismita Nandy, Sunil Kumar Khare and Sangeeta Negi (2012). Bioremediation of waste cooking oil using a novel lipase produced by Penicillium chrysogenum SNP5 grown in solid medium containing waste grease, Bioresource Technology, 120, 300–304 24. Siddharth Vats, Rajesh kumar and Sangeeta Negi (2012), Natural food that meet antibiotics resistance challenges: Invitro synergitics antimicrobial activity of Azadirachta indica, Terminalia chebula, Piper nigrum and photoactivated cow urine. Asian Journal of Pharmaceutical and Biological Research, 2(2), 122-126 25. Sangeeta Negi and Rintu Banerjee (2011), Extraction and purification of glucoamylase and protease produced by Aspergillus awamori in a Single Fermentation, Food Technology and Biotechnology, 49 (3), 310–315 26. Sunil kumar., Nitin Katiyar., Priyanka Ingle., Sangeeta Negi (2011), Optimization of Lipase production using grease waste as substrate through EVOP-factorial Design Technique. Bioresourse Technology, 102, 4909-4912 27. Sangeeta Negi and Rintu Banerjee (2010), Study of conformational changes in glucoamylase of Aspergillus awamori nakazawa in presence of denaturants through CD-spectroscopy. Bioresource Technology, 101(19),7577-7580, 28. Sangeeta Negi and Rintu Banerjee (2010) Optimization of culture parameters to enhance production of amylase and protease from Aspergillus awamori in a single fermentation system, African Journal of Biochemistry Research, 4(3), 73-80 29. Sangeeta Negi and Rintu Banerjee (2009), Optimization of extraction and purification of glucoamylase produced by Aspergillus awamori in Solid-state fermentation, Biotechnology and Bioprocess Engineering, 14, 60-66. 30. Sangeeta Negi and Rintu Banerjee (2009), Characterization of amylase and protease produced by Aspergillus awamori in a single bioreactor, Food Research International, 42(4), 443-448, 31. Sangeeta Negi and Rintu Banerjee (2008), Extraction and purification of protease produced concomitantly with amylase by Aspergillus awamori under SSF, Advances in Life Sciences, .3, 5-10 32. Sangeeta Negi, Veena Pande, R.L.Singh and Laxmi Chand (2007), Studies on seed storage proteins and their synthesis during seed development in pigeon pea, Advances in Life Sciences, 2, 1-5. 33. Sangeeta Negi and Rintu Banerjee (2006), Optimization of amylase and protease production from Aspergillu awamori nakazawa in single bioreactor through EVOP factorial design technique, Food Technology Biotechnology, 44(2), 257-261. 34. SP Singh and Sangeeta Negi (1992), Antibacterial and antifungal activities of Mentha arvensis essential oil. Fitoterapia 63(1),76-78 35. SP Singh and Sangeeta Negi (1992), Antibacterial properties of essential oils from Zingiber chryanthum leaves and rhizomes, Fitoterapia 63(1), 73-75.

Conference Proceedings:

Communications in International Conferences: 1. Barnali Hembram and Sangeeta Negi (2020) Microbial Biotransformation of polythene into value added products, published in International Conference on Biotechnological Interventions for Societal Development BioSangam (2020) 21Feb-23Feb2020. 2. Sangeeta Negi, Satypriya, Farha anjum, Ananad Kumar (2019) Process Optimization for Conversion of Grease Waste in to Fatty Acids by Penicillium chrysogenum SNP5 through Artificial Neural Network (ANN) in International Conference on Innovative Applied Energy (IAPE’19) held at St.Cross Oxford, UK from 14-15 March 2019. Article 040-310, page no:22, ISBN (978-1-912532-05-6). 3. Saangeeta Negi, Satypriya and Farhan Anjum (2018) Sustainable Production of AlkB hydroxylase from Penicillium chrysogenum SNP5 and its characterization in the proceeding of 59th Annual international conference of Association of Microbiologist of India International Symposium of Host pathogen Interaction from Dec 9-12, 2018, at University of Hyderabad. Abstract no.MEP/034 ( 40719) page no.235. 4. Kumari Vibha and Sangeeta Negi (2018) Sustainable production of pectinase from locally isolated Phanerochaete chrysosporium through SSF and its characterization in the proceeding of 59th Annual international conference of Association of Microbiologist of India International Symposium of Host pathogen Interaction from Dec 9-12, 2018, at University of Hyderabad. Abstarct no. IM/038 (40693) page no 505. 5. Sangeeta Negi, Farhan Anjum, Satyapriy and Anand Raj (2018) “Process optimization for the conversion of grease waste in to fatty acid by Penicillium chrysogenumSNP5 under submerged fermentation”. Proceedings of International Conference on Innovational Dimensions: Food, Health and Environmental Biotechnology (Biosangam-2018) organised by department of Biotechnology, MNNIT, Allahabad during 9-11 March2018. 6. Vibha Kumari and Sangeeta Negi (2018) “Synthesis and characterization of Lignin based Nanocomposites with cellulose and Lignin Nanofibers using ultrasonication”. Proceedings of International Conference on Innovational Dimensions: Food, Health and Environmental Biotechnology (Biosangam-2018) organised by department of Biotechnology, MNNIT, Allahabad during 9-11 March2018. 7. S. Negi and K. Anand (2017) Optimization of Alkaline Protease production from Rhizopus oryzae SN-5 through by Artificial Neural Network in an international conference on Emerging Trend in Biotechnology for Waste Conversion (ETWBS-2017) during 8-10 Oct. at NEERI, Nagpur. 8. Sangeeta Negi and Jagat Narayan (2017)“Detoxification of Pine Needle Extract through Fermentation for Therapeutic Use” in an international conference on Emerging Trend in Biotechnology for Waste Conversion (ETWBS-2017) during 8-10 Oct. at NEERI, Nagpur. 9. S.Negi and Farah Anjum (2017) “Enhanced Production of Hydroxylase through Media Engineering from Grease Waste”. in an International Conference on Emerging Trend in Biotechnology for Waste Conversion (ETWBS-2017) during 8-10 Oct. at NEERI, Nagpur. 10. S. Negi, Ayushi Dixit and Ananad Kumar (2017)” Enzyme Processed Paper Recycling Machine Designing”in an International conference on Emerging Trend in Biotechnology for Waste Conversion (ETWBS-2017) during 8-10 Oct. at NEERI, Nagpur. 11. S.Negi and Vibha Kumari (2017) Integrated approach for the synthesis of Lignin based polymer composites from lignin of pine needles biomass” in an international conference on Emerging Trend in Biotechnology for Waste Conversion (ETWBS-2017) during 8-10 Oct. at NEERI, Nagpur. 12. S. Negi, A.K. Pandey, S. Vats, D.P. Maurya, S.P. Singh (2016) An efficient process development for bioethanol production from readily available lignocellulosic biomass waste. In the proceedings of 6th International symposium on “Energy from Biomass and Waste” (Venice 2016), Italy from 14-17 Nov2016. 13. Kumari Vibha and Sangeeta Negi (2016) Exploring Lignocellulolytic enzymes and their properties for the synthesis of lignin based polymers. In proceeding of International conference, on Health, Environment and Industrial Biotechnology (Biosangam -2016)’, has to be organized by Motilal Nehru National Institute of Technology Allahabad, India, 4-6 Februaray, 2016. 14. Neha Choudhary, Shahnaz Khatoon and Sangeeta Negi (2016) Production of Alkane Monooxygenase from grease waste using Penicillium chrysogenum SNP5 in solid state fermentation (SSF). In proceeding of International conference, on Health, Environment and Industrial Biotechnology (Biosangam -2016)’, has to be organized by Motilal Nehru National Institute of Technology Allahabad, India, 4-6 Februaray, 2016. 15. Sapna Jain, Saubhagya Srivastava, Shahnaz Khatoon and Sangeeta Negi (2016) Optimization of Process parameters and partial purification of proteases from locally isolated Rhizopus oryzae SN-1 strain. In proceeding of International conference, on Health, Environment and Industrial Biotechnology (Biosangam -2016)’, has to be organized by Motilal Nehru National Institute of Technology Allahabad, India, 4-6 Februaray, 2016. 16. Ajay Kumar Pandey and Sangeeta Negi (2016) Activated Charcoal mediated conditioning of Sorghum Stover hydrolyzate for improved ethanol production by Saccharomyces cerevisiae NCIM 3288. In proceeding of International conference, on Health, Environment and Industrial Biotechnology (Biosangam -2016)’, organized by Motilal Nehru National Institute of Technology Allahabad, India, 4-6 February, 2016. 17. Farhan Anjum and Sangeeta Negi (2015) Biosurfactant production by locally isolated Bacillus sp. MTCC 5877 and its multifarious applications. In proceeding of International Conference, on New Horizons in Biotechnology, 2015 November 22-25, Trivandrum, Kerala, India. 18. Ajay Kumar Pandey and Sangeeta Negi (2015) Optimization of Cellulase and Xylanase Production in Single Bioreactor through SSF by EVOP Factorial Design by using Locally Isolated Rhizopus oryzae SN-1 strain. In proceeding of International Conference, on New Horizons in Biotechnology, 2015 November 22-25, Trivandrum, Kerala, India. 19. Ajay Kumar Pandey, Sudheer Rai and Sangeeta Negi (2015) Sequential dilute alkali and acid pretreatment of Pine Forest Litter for Bio-ethanol production by Saccharomyces cerevisiae NCIM 3288. In Proceeding of The International Conference on Solid Wastes 2015: Knowledge Transfer for Sustainable Resource Management, organized by Sino-Forest Applied Research Centre for Pearl River Delta Environment (ARCPE), Hong Kong Baptist University, Hong Kong, 19-23 May, 2015. 20. Farhan Anjum, Gunjan Gautam and Sangeeta Negi (2015), A comparative study of bio surfactant production in SSF and SmF by using locally Isolated Penicillium Chrysogenum SNP5 and Bacillus Sp.MTCC 5877. In Proceeding of The International Conference on Solid Wastes 2015: Knowledge Transfer for Sustainable Resource Management, organized by Sino-Forest Applied Research Centre for Pearl River Delta Environment (ARCPE), Baptist University, Hong Kong, 19-23 May, 2015. 21. Ajay Kumar Pandey, Sudheer Rai and Sangeeta Negi (2014) Physicochemical characterization of alkali pretreated Pine foliages and evaluation of Immobilized Cellulases for enhanced saccharification and enzyme recyclability. In proceedings of Indo-US Conference on Advanced Lignocellulosic Biofuels (IUSCALB-2014) organized by IICT, Hyderabad from 10-11 November, 2014. 22. Sudheer Rai, Ajay Kumar Pandey, Sangeeta Negi. “Enhanced detoxification of sorghum Stover hydrolysate by combined action of overliming & Amberlite IRA 400.” was presented in an International conference Asian Congress on Biotechnology (ACB2013) organized by IIT Delhi from 15-19 December 2013 in New Delhi. 23. Ajay Kumar Pandey, Devendra Prasad Maurya, Siddharth Vats, Sudheer Rai and Sangeeta Negi. A comparative study of surfactant assisted acid and alkaline pretreatment of pine foliages for improved transformation of lignocellulosic into fermentable sugars, In proceeding of international conference advances in biotechnology and bioinformatics. ICABB-2013. Organised by Dr D.Y. Patil Vidyapeeth, Pune from 25-27 November, 2013. 24. Siddharth Vats, Jayanti Kumari, Siddharth Vats, Devendra Prasad Maurya Sudheer Rai, Suneel Kumar and Sangeeta Negi. Optimization of process variables for laccase biosynthesis from Pleurotus ostreatus MTCC 1802 using Evolutionary Optimization Techniques. In proceeding of international conference advances in biotechnology and bioinformatics. ICABB-2013. Organised by Dr D.Y. Patil Vidyapeeth, Pune from 25-27 November, 2013. 25. Devendra Prasad maurya, Siddharth Vats, Suneel Kumar, Ajay Pandey and Sangeeta Negi, OPtimizaton of acid pretreatment of sugar cane top for bioethanol production. International Conference on Health, Environment and Industrial Biotechnology (BioSangam 2013) November 21-23. Motilal Nehru National Institute of Technology, Allahabad. 26. Siddharth Vats, Suneel Kumar, Ajay Pandey and Sangeeta Negi. Study and analysis of medicinally valuable phytochemicals from Pinus Roxburghii forest wastes for health care benefits. International Conference on Health, Environment and Industrial Biotechnology (BioSangam 2013) November 21-23. Motilal Nehru National Institute of Technology, Allahabad. 27. Gunjan Gautam, Siddharth Vats, Devendra Prasad Maurya, Charvi Raman, Ajay Pandey, Sudheer Rai and Sangeeta Negi. Production of enzymes from locally isolated microbial consortium SNS-5 to release sugars from agricultural wastes residues. International Conference on Health, Environment and Industrial Biotechnology (BioSangam 2013) November 21-23. Motilal Nehru National Institute of Technology, Allahabad. 28. Sudheer Rai, Ajay Pandey, Siddharth Vats, Devendra Prasad Maurya, Sangeeta Negi. Double pretreatment of sorghum Stover with combined process using dilute sulfuric acid and sodium hydroxide. International Conference on Health, Environment an